To invent new biotechnologies, we develop computational platforms to survey the natural metagenomic diversity and mine for novel enzyme mechanisms. Upon discovering evolutionary innovations from the phage-host arms race, we leverage microbial genomics, functional biochemistry, and ML-guided effector engineering to create new bioengineering tools. Recent work from the lab uncovered highly efficient systems for programmable transcriptome engineering (Cas13) and targeted genomic integration of large payloads (DNA integrases).
We aim to push the boundaries of synthetic biology in human cells via genome, epigenome, transcriptome, and protein engineering. First, we think backwards from major unmet therapeutic needs and then develop platform solutions that enable new kinds of genetic manipulations. Current areas of interest include cell type-specific control of biological perturbations, turning the dial on epigenetic memory, and manipulation of RNA splicing.
Matthew G. Durrant*, Nicholas T. Perry*, James J. Pai, Aditya R. Jangid, Januka S. Athukoralage, Masahiro Hiraizumi, John P. McSpedon, April Pawluk, Hiroshi Nishimasu, Silvana Konermann, Patrick D. Hsu
Matthew G. Durrant, Alison Fanton, Josh Tycko, Michaela Hinks, Sita S. Chandrasekaran, Nicholas T. Perry, Julia Schaepe, Peter P. Du, Peter Lotfy, Michael C. Bassik, Lacramioara Bintu, Ami S. Bhatt & Patrick D. Hsu
Sita S. Chandrasekaran, Shreeya Agrawal, Alison Fanton, Aditya R. Jangid, Bérénice Charrez, Arturo M. Escajeda, Sungmin Son, Roger Mcintosh, Huyen Tran, Abdul Bhuiya, María Díaz de León Derby, Neil A. Switz, Maxim Armstrong, Andrew R. Harris, Noam Prywes, Maria Lukarska, Scott B. Biering, Dylan C. J. Smock, Amanda Mok, Gavin J. Knott, Qi Dang, Erik Van Dis, Eli Dugan, Shin Kim, Tina Y. Liu, IGI Testing Consortium, Erica A. Moehle, Katherine Kogut, Brenda Eskenazi, Eva Harris, Sarah A. Stanley, Liana F. Lareau, Ming X. Tan, Daniel A. Fletcher, Jennifer A. Doudna, David F. Savage & Patrick D. Hsu
Scott B. Biering, Sylvia A. Sarnik, Eleanor Wang, James R. Zengel, Sarah R. Leist, Alexandra Schäfer, Varun Sathyan, Padraig Hawkins, Kenichi Okuda, Cyrus Tau, Aditya R. Jangid, Connor V. Duffy, Jin Wei, Rodney C. Gilmore, Mia Madel Alfajaro, Madison S. Strine, Xammy Nguyenla, Erik Van Dis, Carmelle Catamura, Livia H. Yamashiro, Julia A. Belk, Adam Begeman, Jessica C. Stark, D. Judy Shon, Douglas M. Fox, Shahrzad Ezzatpour, Emily Huang, Nico Olegario, Arjun Rustagi, Allison S. Volmer, Alessandra Livraghi-Butrico, Eddie Wehri, Richard R. Behringer, Dong-Joo Cheon, Julia Schaletzky, Hector C. Aguilar, Andreas S. Puschnik, Brian Button, Benjamin A. Pinsky, Catherine A. Blish, Ralph S. Baric, Wanda K. O’Neal, Carolyn R. Bertozzi, Craig B. Wilen, Richard C. Boucher, Jan E. Carette, Sarah A. Stanley, Eva Harris, Silvana Konermann & Patrick D. Hsu
Cheng Zhang, Silvana Konermann, Nicholas J. Brideau, Peter Lotfy, Xuebing Wu, Scott J. Novick, Timothy Strutzenberg, Patrick R. Griffin, Patrick D. Hsu, Dmitry Lyumkis
Preprint on bioRxiv
Silvana Konermann, Peter Lotfy, Nicholas J. Brideau, Jennifer Oki, Maxim N. Shokhirev, Patrick D. Hsu
Highlighted in Nature Methods, The CRISPR Journal, Alzforum
Patrick D. Hsu, Eric S. Lander, Feng Zhang
Patrick D Hsu, David A Scott, Joshua A Weinstein, F Ann Ran, Silvana Konermann, Vineeta Agarwala, Yinqing Li, Eli J Fine, Xuebing Wu, Ophir Shalem, Thomas J Cradick, Luciano A Marraffini, Gang Bao & Feng Zhang
F. Ann Ran, Patrick D. Hsu, Chie-Yu Lin, Jonathan S. Gootenberg, Silvana Konermann, Alexandro E. Trevino, David A. Scott, Azusa Inoue, Shogo Matoba, Yi Zhang, Feng Zhang
Nature Biotechnology
Nature Biomedical Engineering
Nature Genetics
Nature Chemical Biology
Patrick Hsu is Co-Founder of the Arc Institute and Assistant Professor of Bioengineering and Deb Faculty Fellow at the University of California, Berkeley. The Hsu lab develops biotechnologies to improve human health through next-generation diagnostics and therapeutics, recently reporting platforms for gene writing with DNA integrases, programmable RNA targeting, and point-of-care CRISPR diagnostics. Patrick received A.M. and Ph.D. degrees from Harvard University and the Broad Institute, where he was an early pioneer of CRISPR-Cas9 technologies for genome editing. His research has been recognized by MIT Technology Review's Innovators Under 35, the Amgen Young Investigator Award, Forbes' 30 Under 30, the NIH Early Independence Award, and the Rainwater Prize for Innovative Early Career Scientist.
Massachusetts General Hospital/Harvard Medical School (Postdoc) & University of Wisconsin-Madison (PhD in Biochemistry)
Stanford (Postdoc) & Open University of London (PhD)
Stanford University (Postdoc) & University of Toronto (PhD in Pathobiology)
UC Berkeley (Postdoc) & Stanford University (PhD in Genetics)
UCSF (Postdoc) & University of St Andrews (PhD in Biochemistry)
PhD, Bioengineering, UC San Diego
Seoul National University (PhD in Biophysics)
UC Berkeley Chemistry PhD Program
Soros Fellow, UC Berkeley-UCSF Joint PhD Program in Bioengineering
NSF Fellow, UC Berkeley-UCSF Joint PhD Program in Bioengineering
UC Berkeley-UCSF Joint PhD Program in Bioengineering
UC Berkeley-UCSF Joint PhD Program in Bioengineering
UC Berkeley Molecular and Cell Biology PhD Program
B.S.E Chemical and Biomolecular Engineering, University of Pennsylvania
B.S. Biochemistry, M.S. Cell & Molecular Biology, UCSD
B.S. Bioengineering, B.S. EECS, UC Berkeley
UC Berkeley Bioengineering
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